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| Molecular
Diagnostic Techniques |
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| Nucleic Acid or Protein Technique |
Notes |
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histochemical stains |
the classical stains in pathology labs |
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IHC (immunohistochemical) stains |
large #s of markers & available in many community labs |
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cytochemical stains (leukemia Dx) |
classical stains in hematology & cytopathology labs |
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electrophoresis |
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| PCR (polymerase chain reaction DNA amplification) |
Roche Diagnostics |
| RT-PCR (reverse transcriptase PCR) |
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| TMA (transcription-mediated DNA amplification) |
Gen-Probe |
| LCR (ligase chain reaction DNA amplification) |
Abbott Laboratories |
| bDNA (branched-chain DNA) |
Bayer |
| FISH (fluorescent in-situ hybridization) |
Vysis/Abbott |
| CISH (chromogenic in-situ hybridization) |
Ventanna |
| DNA Sequencing |
Visible Genetics Applied Biosystems Beckman Coulter |
| SDA (strand displacement amplification) |
Becton Dickinson |
| Dot blot (DNA Dot-blot Hybridization) |
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| Slot blot |
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| SB (immunoblot...Southern Blot) |
a labled DNA method in 1970s by E. M. Southern to
detect DNA segments |
| NB (immunoblot...northern Blot) |
a labled DNA method to detect mRNA segments |
| WB (immunoblot...western Blot) |
a labled antibody method to detect specific cell
proteins |
| SWB (immunoblot...southwestern Blot) |
a labled DNA probe method to detect specific cell proteins |
| EITB (enzyme-linked
immunotransfer blot assay) |
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- See Wikipedia on-line
community-created encyclopedia, beginning with category of "science",
then "laboratory", then "laboratory techniques".
(posted 2004; latest addition 22 July 2005) |
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© Copyright
1999 - 2006, all rights reserved, Pathology Associates Of Lexington,
P.A. |
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